BioPaddles® - Nutrient Agar (NA)

Order Code: 5550

5550 200x200

The BioPaddles® NA/NA can be used for the cultivation of a wide variety of non-fastidious bacteria.

Nutrient Agar (NA)

The nitrogen, carbon, vitamins, and amino acids in Nutrient Agar are provided by enzymatic digest of gelatin and beef extract. Agar and a proprietary polymer are the solidifying agents.

Application

In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing.1 Nutrient Agar continues to be a widely used general purpose medium for growing non-fastidious microorganisms.

If required, enrichments can be added to this medium. Nutrient Agar meets APHA and Association of Official Analytical Chemists (AOAC) standard methods.Nutrient Agar is specified in Standard Methods for the Examination of Water and Wastewater procedures for the examination of food, dairy products, water, and other materials.

 

Specifications

Storage/Expiration

Store tightly sealed BioPaddles® in a cool, dry location. Shield from direct sunlight. Store BioPaddles® at room temperature (65 to 77°F/18 to 25°C). Avoid sudden temperature changes. Temperature fluctuations may result in condensation settling at the bottom of the vial. This will not affect the culture properties, but could reduce the shelf-life or cause the agar to separate from the plastic paddle support. Do not refrigerate. Refrigeration may result in water condensation. Avoid freezing. If freezing occurs, thaw for 3-6 hours in the refrigerator (40°F; 4.4°C). Freezing can promote excess water loss and variation in media surface due to crystal formation. Refer to Best Before End date (See: BBE stamped on vial). Discard if paddle agar appears oxidized and darker than the expected color or if contaminants appear. The expiration date is based on medium in an intact container that is stored as directed.

Sampling

Liquids: Twist to remove paddle from vial. Fill vial to 40 mL fill line with the liquid to be sampled. The 40 mL volume can be used to calculate Total Viable Count (TVC) and/or Total Colony Count (TCC). Replace paddle. Allow a contact time of 15 seconds. Remove the paddle. Empty the vial. Replace the paddle in the vial.

Surfaces: Twist to remove paddle from vial. Allow the paddle surface (10 cm2) to come into physical contact with the test surface. Recovery rate is about 50%. To ensure an accurate recovery, gently sweep (or touch) the paddle to cover a 20 cm2 area. Replace paddle in vial.

Incubation

Temperature Minimum Period Optimal Period
35°C (bacteria) 72 hours 5-7 days
20-25°C (fungi) 5 days 7 days

Colony Measuring

Each BioPaddles® paddle has molded media attachment points that are 4mm
in length (point-to-point). This feature provides a useful guidepost to estimating nearby colony size.

Disposal

Twist to remove paddle from vial. Fill vial to 40 mL fill line with 1:9 dilution of household bleach (5.25% sodium hypochlorite). Replace paddle in vial. Allow 15 minute contact time. Remove paddle. Discard bleach solution. Replace paddle in vial and dispose. Alternatively, loosen cap and microwave for 30 seconds, autoclave, or incinerate.

Technical Documents

 

 

BioPaddle Apps

 

 


Free Lite App

BioPaddlesLite
 

BioPaddles® Colony ID™ lite

Description

The BioPaddles® Colony ID™ lite app allows the user to presumptively identify microbe colonies on LaMotte BioPaddles® by comparing them to a library of 80 standard images covering 5 microhabitats (AIR, WATER, SOIL, SURFACE, and FOOD) and 9 paddle agar types. The image series includes side views, and top views at the colony and cell level.

Resource materials include instructions for use of the BioPaddles®, detailed fact sheets for each agar type and microbe genera (along with selected species) – including microhabitat preferences, procedures for common microbiological techniques, and examples of microbe growth on BioPaddles® from a variety of microhabitat sources.

 

View In iTunes

BioPaddles® Colony ID™ lite Tutorial PDF

 

biopaddles full app
 

BioPaddles® Colony ID™

Description

The BioPaddles® Colony ID™ includes over 250 standard images covering 30+ microbe genera in five microhabitats (AIR, WATER, SOIL, SURFACE, and FOOD) on 9 paddle agar types. The image series includes side views and top views at the colony and cell level.

New camera and reporting functions allow the user to import digital images and compare their samples to know standards. It also allows the user to create and email reports.

View In iTunes

BioPaddles® Colony ID™ Tutorial PDF